Journal: Lipids in Health and Disease

Article Title: Nitroxoline mitigates hepatic steatosis by enhancing cholesterol efflux and promoting bile acid synthesis through LRH-1 signaling

doi: 10.1186/s12944-025-02720-5

Figure Lengend Snippet: Nitroxoline regulates ABCG8, ABCG5 and CYP7A1 expression in Ldlr −/− mice fed with a HFD. A The mRNA levels of ABCA1, ABCG1, ABCG5, ABCG8, SR-B1, NPC1L1, CPT1A and CYP7A1 of mouse liver tissues were assessed by qPCR analysis ( n = 3). B The protein levels of ABCG5, ABCG8, CYP7A1 and GAPDH were determined by western blot analysis ( n = 3). Data was presented as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.005, **** P < 0.001, compared with the vehicle group by Student’s t-test

Article Snippet: Anti-ABCG5 antibody (Cat. No: 27722–1-AP, RRID: AB_2880952) was sourced from Proteintech, Rosemont (IL), USA.

Techniques: Expressing, Western Blot

Journal: Lipids in Health and Disease

Article Title: Nitroxoline mitigates hepatic steatosis by enhancing cholesterol efflux and promoting bile acid synthesis through LRH-1 signaling

doi: 10.1186/s12944-025-02720-5

Figure Lengend Snippet: Nitroxoline enhances ABCG5, ABCG8 and CYP7A1 expression in vitro. Huh7 cells incubated with LPDS (5%) were treated with Nit (5 μM) and the solvent vehicle for 24 h. A The mRNA levels of ABCA1, ABCG1, ABCG5, ABCG8, SR-B1, NPC1L1, CPT1A and CYP7A1 were assessed by qPCR analysis ( n = 3). Huh-7 cells incubated with LPDS (5%) were treated with various doses of Nit or solvent vehicle for 24 h. B The expression of ABCG5, ABCG8 and CYP7A1 at the mRNA levels was determined by qPCR. C The protein levels of ABCG5, ABCG8, CYP7A1 and GADPH were determined by western blot analysis (n = 3). Data was presented as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.005, compared with the vehicle group by Student’s t-test

Article Snippet: Anti-ABCG5 antibody (Cat. No: 27722–1-AP, RRID: AB_2880952) was sourced from Proteintech, Rosemont (IL), USA.

Techniques: Expressing, In Vitro, Incubation, Solvent, Western Blot

Journal: Lipids in Health and Disease

Article Title: Nitroxoline mitigates hepatic steatosis by enhancing cholesterol efflux and promoting bile acid synthesis through LRH-1 signaling

doi: 10.1186/s12944-025-02720-5

Figure Lengend Snippet: Nitroxoline preserved the protein expression of ABCG8 and CYP7A1. Huh-7 cells were incubated with Nit (5 μM) and the solvent vehicle and treated with for Act. D (5 μM) indicated period. A The expression of ABCG5, ABCG8 and CYP7A1 at the mRNA levels was determined by qPCR analysis. B Huh-7 cells were incubated with Nit (5 μM) and the solvent vehicle and treated with for CHX (5 μM) indicated period. Data were presented as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, compared with the vehicle group by Student’s t-test

Article Snippet: Anti-ABCG5 antibody (Cat. No: 27722–1-AP, RRID: AB_2880952) was sourced from Proteintech, Rosemont (IL), USA.

Techniques: Expressing, Incubation, Solvent

Journal: Lipids in Health and Disease

Article Title: Nitroxoline mitigates hepatic steatosis by enhancing cholesterol efflux and promoting bile acid synthesis through LRH-1 signaling

doi: 10.1186/s12944-025-02720-5

Figure Lengend Snippet: Nitroxoline modulated ABCG5, ABCG8 and CYP7A1 expression through LRH-1 signaling. A The heatmap plot shows the differential expression of RNA-seq analysis in Nit-treated and untreated Huh-7 cells. The values of three independent experiments were transformed into z scores for comparison. The color scale indicates high (red), average (white), and low (blue) values. B Huh-7 cells were transfected with 200 ng LRH-1 response element reporter plasmid and then, pretreated with/without LRH-1 inhibitor, ML-180 (5 μM), followed by Nit treatment. Promoter activities were detected using the Dual-Luciferase Reporter Assay System. C Huh 7 cells were incubated with LPDS (5%) were pre-treated with/without ML-180 (5 μM), then incubated with the solvent vehicle and/or Nit (5 μM) for 24 h. The mRNA levels of LRH-1, CYP7A1, ABCG5 and ABCG8 were assessed by qPCR analysis (n = 3). D The protein levels of ABCG5, ABCG8, CYP7A1 were determined by western blotting. GAPDH served as a loading control. E The mRNA and ( F ) protein level of LRH-1 of mouse liver tissues were assessed by qPCR analysis ( n = 3). Data was presented as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.005, compared with the vehicle group by Student’s t-test

Article Snippet: Anti-ABCG5 antibody (Cat. No: 27722–1-AP, RRID: AB_2880952) was sourced from Proteintech, Rosemont (IL), USA.

Techniques: Expressing, Quantitative Proteomics, RNA Sequencing, Transformation Assay, Comparison, Transfection, Plasmid Preparation, Luciferase, Reporter Assay, Incubation, Solvent, Western Blot, Control

Journal: Open Life Sciences

Article Title: Polydatin prevents cholesterol gallstone formation by regulating cholesterol metabolism via PPAR-γ signaling

doi: 10.1515/biol-2022-1009

Figure Lengend Snippet: Polydatin regulates cholesterol metabolism-associated genes and activates PPAR-γ signaling in mice. (a) ABCG5, ABCG8, CYP7A1, and MUC5AC levels in liver tissues were detected by real-time PCR. (b) ABCG5, ABCG8, CYP7A1, and MUC5AC protein levels in liver tissues were determined by western blotting. (c) The levels of PPAR-γ in the cytoplasm and nucleus of liver tissues were measured by western blotting. ## P < 0.01 vs control group. ** P < 0.01 vs LD group.

Article Snippet: The membranes were incubated with primary antibodies against ABCG5 (1:500; 27722-1-AP; Proteintech group, USA), ABCG8 (1:1,000; ab223056; Abcam, USA), CYP7A1 (1:1,000; bs-21429R; Bioss, China), MUC5AC (1:1,000; ab3649; Abcam), or PPAR-γ (1:1,000; ab45036; Abcam) at 4°C overnight.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: Open Life Sciences

Article Title: Polydatin prevents cholesterol gallstone formation by regulating cholesterol metabolism via PPAR-γ signaling

doi: 10.1515/biol-2022-1009

Figure Lengend Snippet: Polydatin modulates cholesterol metabolism-related genes and activates PPAR-γ signaling in LPS-exposed HIBECs. (a) ABCG5, ABCG8, CYP7A1, and MUC5AC levels were measured by real-time PCR. (b) ABCG5, ABCG8, CYP7A1, and MUC5AC protein levels were detected by western blotting. (c) Cytoplasmic and nuclear levels of PPAR-γ were determined by western blotting. (d) Translocation of PPAR-γ was detected by immunofluorescence assay. Scale bars represent 25 μm. ## P < 0.01 vs control group. ** P < 0.01 vs LPS group.

Article Snippet: The membranes were incubated with primary antibodies against ABCG5 (1:500; 27722-1-AP; Proteintech group, USA), ABCG8 (1:1,000; ab223056; Abcam, USA), CYP7A1 (1:1,000; bs-21429R; Bioss, China), MUC5AC (1:1,000; ab3649; Abcam), or PPAR-γ (1:1,000; ab45036; Abcam) at 4°C overnight.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Translocation Assay, Immunofluorescence, Control

Journal: Food Science & Nutrition

Article Title: Age‐Independent Hypocholesterolemic Effects of Rice Protein via FXR ‐Mediated Stimulation of Cholesterol Elimination

doi: 10.1002/fsn3.70687

Figure Lengend Snippet: Hepatic mRNA levels and protein expressions of ABCG5 in growing and adult rats. (A) Hepatic mRNA levels of ABCG5; (B) Hepatic protein expressions of ABCG5. Values are the means ± SEM ( n = 6). * p < 0.05, in comparison with CAS‐G. ** p < 0.05, in comparison with CAS‐A. ABCG5, ATP‐binding cassette transporter G5; CAS‐A, adult rats fed with casein for 2 weeks; CAS‐G, growing rats fed with casein for 2 weeks; RP‐A, adult rats fed with rice protein for 2 weeks; RP‐G, growing rats fed with rice protein for 2 weeks.

Article Snippet: Afterward, the membranes were incubated with a blocking solution of 5% non‐fat milk in TBS at room temperature for 1 h. They were then subjected to overnight incubation at 4°C with primary antibodies targeting ABCG1 (Proteintech, Wuhan, China), ABCG5 (Proteintech, Wuhan, China), BSEP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), coactivator‐associated arginine methyltransferase 1 (CARM1, Proteintech, Wuhan, China), FXR (Proteintech, Wuhan, China), SR‐BI (Proteintech, Wuhan, China), and β‐actin (Cell Signaling, Danvers, MA, USA).

Techniques: Comparison, Binding Assay